INVESTIGATION OF EXTENDED SPECTRUM BETA-LACTAMASE AND CARBAPENEMASE PRODUCING ENTEROBACTERALES STRAINS IN GREEN VEGETABLES

نویسندگان

چکیده

Çanakkale semt pazarlarında satılan 96 adet yeşil sebze örneğinde “beta laktam” dirençli Enterobacterales suşlarının varlığı araştırılmıştır. Gövde, yaprak kısımlarından alınan örneklerden Violet Red Bile Glucose (VRBG) Agara ekilmiştir. yükleri <1 log KOB/g ile 6.11 arasında tespit edilmiştir. Kromojenik GSBL Agar ve 2 mg/ml meropenem içeren EMB Agarda üreyen 129 izolat MALDI-TOF MS yöntemine göre Serratia liquefaciens (n=35), plymuthica (n=31), Klebsiella oxytoca (n=25), pneumonia (n=6), Raoultella ornithinolytica (n=14), terrigena (n=3), Enterobacter cloacae (n=11), ludwigii (n=4), asburiae (n=1), Lelliottia amnigena (n=16), Escherichia coli (n=2), hermannii Leclercia adecarboxylata Pantoea agglomerans Kluyvera intermedia Kosakonia cowanii (n=1) Hafnia alvei olarak tanımlanmıştır. Disk difüzyon izolatların %11.62’sinin (n=15) Genişlemiş Spektrumlu “Beta Laktamaz” (GSBL), %4.65’inin (n=6) karbapenemaz pozitif olduğu belirlenmiştir. Yeşil sebzelerde karbapenem ısıl işlem görmeden tüketilen bu gıdalardaki halk sağlığı riskini ortaya koymaktadır.

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ژورنال

عنوان ژورنال: Gida the Journal of Food

سال: 2023

ISSN: ['1309-6273', '1300-3070']

DOI: https://doi.org/10.15237/gida.gd22112